Tawaran Penelitian Hibah Penelitian Mahasiswa Departemen Gizi Kesehatan FK-UGM 2017
| Agenda | Jadwal |
|---|---|
| Batas Akhir Penerimaan Proposal | Senin, 6 Maret 2017 |
| Seleksi Proposal | Senin-Jumat, 13-17 Maret 2017 |
| BPengumuman Proposal lolos seleksi | Senin, 27 Maret 2017 |
Siti Helmyati1,Mohammad Juffrie2,Endang Sutriswati Rahayu3,BJ. Istiti Kandarina4
1Department of Nutrition, Faculty of Medicine, UGM, Yogyakarta
2Department of Child Health, General Hospital Dr. Sardjito, Yogyakarta, Indonesia
3Faculty of Food Technology, UGM, Yogyakarta
4Department of Public Health, Faculty of Medicine, UGM, Yogyakarta
ABSTRACT
The role of gut microbiota in human health is highly understandable. Recent research highlights the influence of geographical terms in gut microbiota composition. Unfortunately, baseline data of microbiota composition in children are limited. We compared the number of fecal microbiota of children, ages 9-12 years, living in Kulon Progo (KP), Yogyakarta with same age children in West Lombok (WL), West Nusa Tenggara. The study was an observational study with comparative design.The subjects were elementary school students, 29 subjects in KP and 27 subjects in WL. Fecal microbiota was analyzed by pour plate culture technique on MRS agar for lactic acid bacteria (LAB) and TBX agar for E. coli. The difference of total bacteria was tested using independent sample t-test. Mean of total fecal LAB of children in KP was 7.58±0.61 CFU/g, whereas in WL was 7.19±0.83 CFU/g. There is no significant difference between the number of fecal LAB of children in KP and in WL (p=0.255). Mean of total fecal E. coli of children in KP was 7.06±0.75 CFU/g, whereas in WL was 7.23±1.12 CFU/g. There is no significant difference between the number of fecal E. coli of children in KP and in WL (p=0.078). Further research is needed to determine the factors that can affect the composition of the gut microbiota.
Susetyowati,
Department of Nutrition and Health, Faculty of Medicine Gadjah Mada University,
Farmako Street, Sekip Yogyakarta 55281, e-mail: susetyowati@ugm.ac.id
Background.
Malnutrition in hospitalized patients is considered as a significant problem, thus nutritional screening should be performed in all patient on hospital admission.In Indonesia was developed nutrition screening tool, namely the Simple Nutrition Screening Tool (SNST) which was valid and reliable. The objective of this study was to compare the SNST and Nutrition risk screening-2002 (NRS-2002) against the Subjective Global Assessment in patients admitted to Sleman District Hospital.
Siti Helmyati1*, Narendra Yoga Hendarta2, Endri Yuliati1
1Department of Nutrition, Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia
2Health Analyst, Polytechnic of Health, Ministry of Health, Yogyakarta, Indonesia
*Corresponding author: siti_helmyati@yahoo.com
Abstract
Iron (Fe) and zinc (Zn) are essential for human. The addition of Fe intake, however has negative effect on gut microbiota balance, which increases gut pathogens. Probiotics are able to maintain digestive tract health while prebiotics can improve the absorption of minerals. It was needed to test the effect of synbiotic fermented milk on the gut microbiota. Synbiotic fermented milk with Lactobacillus plantarum Dad-13 and Fructo-oligosaccharides (FOS) was double fortified with 100 ppm Fe and 50 ppm Zn. Wistar rats were divided into 3 groups and given: 1) synbiotic fermented milk with NaFeEDTA and Zn acetate (NA), 2) with Fe gluconate and Zn acetate (FE) and 3) without fortification (KO) every day for 1 months. Total microflora (lactobacilli, bifidobacteria, enterobacteria, Escherichia coli) were measured after intervention using selective medium agar. The number of E. coli differed significantly in NA and FE, namely 4.976 and 6.456 log CFU/g digesta, respectively (p <0.05). KO had the highest number of lactic acid bacteria (lactobacilli and bifidobacteria) but the number of lactobacilli, bifidobacteria and enterobacteria did not significantly differ among three groups (p> 0.05). Iron fortification has to be done carefully because it can increase E. coli. It is necessary to use iron compounds that bound to other compounds such as NaFeEDTA.
Lily Arsanti Lestari1,*,Marsetyawan HNE Soesatyo2, Susi Iravati3, Eni Harmayani4
1Department of Nutrition and Health, Faculty of Medicine, Gadjah Mada University, Jl. Farmako, Sekip Utara, Yogyakarta, Indonesia
2Department of Cell Biology and Histology, Faculty of Medicine, Gadjah Mada University, Jl. Farmako, Sekip Utara, Yogyakarta, Indonesia
3Department of Microbiology, Faculty of Medicine, Gadjah Mada University, Jl. Farmako, Sekip Utara, Yogyakarta, Indonesia
4Department of Agricultural Product Technology, Faculty of Agricultural Technology, Jl. Flora, Bulaksumur, Yogyakarta, Indonesia
* Corresponding author: lily_al@ugm.ac.id / santi_wap@yahoo.com
