Siti Helmyati1,Mohammad Juffrie2,Endang Sutriswati Rahayu3,BJ. Istiti Kandarina4
1Department of Nutrition, Faculty of Medicine, UGM, Yogyakarta
2Department of Child Health, General Hospital Dr. Sardjito, Yogyakarta, Indonesia
3Faculty of Food Technology, UGM, Yogyakarta
4Department of Public Health, Faculty of Medicine, UGM, Yogyakarta
The role of gut microbiota in human health is highly understandable. Recent research highlights the influence of geographical terms in gut microbiota composition. Unfortunately, baseline data of microbiota composition in children are limited. We compared the number of fecal microbiota of children, ages 9-12 years, living in Kulon Progo (KP), Yogyakarta with same age children in West Lombok (WL), West Nusa Tenggara. The study was an observational study with comparative design.The subjects were elementary school students, 29 subjects in KP and 27 subjects in WL. Fecal microbiota was analyzed by pour plate culture technique on MRS agar for lactic acid bacteria (LAB) and TBX agar for E. coli. The difference of total bacteria was tested using independent sample t-test. Mean of total fecal LAB of children in KP was 7.58±0.61 CFU/g, whereas in WL was 7.19±0.83 CFU/g. There is no significant difference between the number of fecal LAB of children in KP and in WL (p=0.255). Mean of total fecal E. coli of children in KP was 7.06±0.75 CFU/g, whereas in WL was 7.23±1.12 CFU/g. There is no significant difference between the number of fecal E. coli of children in KP and in WL (p=0.078). Further research is needed to determine the factors that can affect the composition of the gut microbiota.
Keywords: child health, escherichia coli, lactic acid bacteria, gut microbiota, Indonesian children
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