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Immunomodulatory Effect of Lactobacillus plantarum Mut7 and Sweet Potato Fiber

Lily Arsanti Lestari1,*,Marsetyawan HNE Soesatyo2, Susi Iravati3, Eni Harmayani4

1Department of Nutrition and Health, Faculty of Medicine, Gadjah Mada University, Jl. Farmako, Sekip Utara, Yogyakarta, Indonesia
2Department of Cell Biology and Histology, Faculty of Medicine, Gadjah Mada University, Jl. Farmako, Sekip Utara, Yogyakarta, Indonesia
3Department of Microbiology, Faculty of Medicine, Gadjah Mada University, Jl. Farmako, Sekip Utara, Yogyakarta, Indonesia
4Department of Agricultural Product Technology, Faculty of Agricultural Technology, Jl. Flora, Bulaksumur, Yogyakarta, Indonesia
* Corresponding author: lily_al@ugm.ac.id / santi_wap@yahoo.com

 Abstract

Introduction:Probiotic and prebiotic could affect the immune function both specific and nonspecific immune responses. The objectives of this research were to study the effect indigenous probiotic L. plantarum Mut7 and prebiotic from sweet potato fiber (SPF) on phagocytic activity and NO production of peritoneal macrophage, concentration of intestinal fluid sIgA, and cytokines (IFN-g, TGF-b, IL-4) in the supernatant of Peyer’s Patch.

Methods:Ninety six Sprague Dawley rats were adapted with AIN-93M diets for 7 days and divided into 2 groups (A–not infected with S. typhimurium and B–infected with S. typhimurium). Each group was divided into 4 sub-groups of 12 rats. The treatment for each sub-group was arranged as follow: 1) AIN-93M diets (CON); 2) AIN-93M diets+L. plantarum Mut7 109 CFU/ml (PRO); 3) modified AIN-93M diets with supplementation of SPF (PRE); 4) PRE diet in combination with PRO treatment (SYN). Collection of peritoneal macrophage, intestinal fluid, and Peyer’s Patch were done after 3 and 5 weeks of treatments.

Results:The results showed that oral administration of PRO, PRE, or SYN could increase phagocytic activity of peritoneal macrophage (P<0.05). PRO individually or together with PRE could induce the nitric oxide production of peritoneal macrophage. The production of sIgA in the intestinal fluid was strongly induced by PRO, PRE, and SYN after 3 and 5 weeks of treatment (P<0.05). The concentration of IFN-gand TGF-b in PP’s supernatant in the group treated with PRO, PRE, and SYN for 5 weeks were higher than CON (P<0.05), however the concentration of IL-4 was lower than CON(P<0.05).

Conclusions:PRO, PRE, and SYN could strongly induce the non-specific immune system by increasing the phagocytic activity and NO production. They also modulate the specific immune system by increasing sIgA in the intestinal fluid and IFN-g and TGF-b in PP’s supernatant.

Keywords probiotic; prebiotic; macrophage; sIgA; cytokines

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